Olympia
| # | Name | Mannschaft | Zeit | |
|---|---|---|---|---|
| 1 |  | Cate Campbell | Cate Campbell | 52.80s |
| 2 |  | Penny Oleksiak | Penny Oleksiak | +0.15s |
| 3 |  | Junxuan Yang | Junxuan Yang | +0.22s |
| 4 | | Kayla Sanchez | Kayla Sanchez | +0.32s |
| 5 |  | Michelle Elizabeth Coleman | Michelle Coleman | +0.73s |
| 6 |  | Freya Anderson | Freya Anderson | +0.81s |
| 7 | | Qingfeng Wu | Qingfeng Wu | +1.07s |
| 8 |  | Federica Pellegrini | Federica Pellegrini | DNS |
