Olympia
| # | Name | Mannschaft | Zeit | |
|---|---|---|---|---|
| 1 |  | Emma McKeon | Emma McKeon | 51.96s |
| 2 |  | Siobhan Haughey | Siobhan Haughey | +0.31s |
| 3 | | Cate Campbell | Cate Campbell | +0.56s |
| 4 |  | Penny Oleksiak | Penny Oleksiak | +0.63s |
| 5 |  | Sarah Sjöström | Sarah Sjöström | +0.72s |
| 6 |  | Femke Heemskerk | Femke Heemskerk | +0.83s |
| 7 |  | Anna Hopkin | Anna Hopkin | +0.87s |
| 8 |  | Abbey Weitzeil | Abbey Weitzeil | +1.27s |
